Abstract Chickens genetically resistant to avian influenza could prevent future outbreaks. In chickens. influenza A virus (IAV) relies on host protein ANP32A. Here we use CRISPR/Cas9 to generate homozygous gene edited (GE) chickens containing two ANP32A amino acid substitutions that prevent viral polymerase interaction. https://www.roneverhart.com/HP-Pavilion-15-eg1053cl-15-6-FHD-Touch-Intel-Core-i5-1235U-3-30-GHz-Intel-Iris-Xe-Graphics-12-GB-DDR4-RAM-512-GB-SSD-/